Researchers studied a toilet with a flushometer that was seeded with C. diff spores in a sealed chamber. They flushed the toilet 24 times and periodically collected postflush bowl water samples and settle plates. They also collected air samples after 12 flushes using rotating plate impactors.
The study shows spores were present in bowl water even after 24 flushes. Additionally, large droplet spore deposition accumulated over the time period of 24 flushes.
Researchers found droplet nuclei spore bioaerosol was produced over at least 12 flushes.
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